Modifying Enzymes
Medchemexpress LLC Hyaluronidase, ovine testes | 37326-33-3 | MFCD00131351 | 95.0% | 55,000 g/mol | 5 MG
Hyaluronidase, ovine testes is a protein enzyme preparation that hydrolyzes hyaluronic acid in extracellular matrices. Supplied as a lyophilized powder, it is intended for use in biochemical assays, tissue dissociation, and reproductive biology research where controlled degradation of hyaluronic acid is required.
- Enzymatic degradation of hyaluronic acid for tissue dissociation and analytic assays.
- Derived from ovine testes for endogenous enzyme activity profile.
- High purity to minimize contaminating proteins and activities (95.0%).
- Average molecular weight approximately 55,000 g/mol for preparative considerations.
- Supplied in small pack sizes suitable for research-scale experiments.
Sigma Aldrich Fine Chemicals Biosciences Ribonuclease A from bovine pancreas for molecular biology, 70Kunitz units/mg protein, lyophilized, 9001-99-4, MFCD00132181
Ribonuclease A from bovine pancreas for molecular biology, 70Kunitz units/mg protein, lyophilized
New England Biolabs, Inc. Endonuclease III (Nth) – 1000 units
Endonuclease III (Nth) protein from E. coli acts as both N-glycosylase and a AP-lyase. The N-glycosylase activity releases damaged pyrimidines from double-stranded DNA, generating a basic (AP site). The AP-lyase activity of the enzyme cleaves 3 to the AP site leaving a 5' phosphate and a 3' ring opened sugar. Some of the damaged bases recognized and removed by Endouclease III include urea, 5, 6 dihydroxythymine, thymine glycol, 5-hydroxy-5 methylhydanton, uracil glycol, 6-hydroxy-5, 6-dihdrothimine and methyltartronylurea.
New England Biolabs, Inc. E.coli DNA Ligase – 1000 units
E. coli DNA Ligase catalyzes the formation of a phosphodiester bond between the 5'-phosphate and the 3'-hydroxyl of two adjacent DNA strands in duplex DNA with cohesive ends. It is not appreciably active on blunt-ended substrates. E. coli DNA Ligase uses NAD as a cofactor and can be heat-inactivated.
New England Biolabs, Inc. DNA Polymerase I (E. coli) – 500 units
DNA Polymerase I (E coli) is a DNA-dependent DNA polymerase with inherent 3' to 5' and 5' to 3' exonuclease activities. The 5' to 3' exonuclease activity removes nucleotides ahead of the growing DNA chain, allowing nick-translation.
INTACT GENOMICS INC Endonuclease IV (Nfo) 5000 Units (10 units/µl)
Endonuclease IV (Nfo) from Escherichia coli is a 32-kD metalloprotein that aids in the repair of damaged DNA. The enzyme functions both as an apurinic/apyrimidinic nuclease (1) and as a 3′-terminal di-esterase (1-4). Its 3′-terminal di-esterase activity is important in the repair of DNA strand breaks generated by oxidation and ionic radiation (2, 3). In such events, the strand breaks terminate with either a 3′ phosphate or a deoxyribose fragment, preventing repair by DNA polymerase I or DNA ligase. Endonuclease IV removes the blocking groups, leaving a free 3′-hydroxyl terminus. This enzyme does not have detectable associated exonuclease or DNA N-glycosylase activity (1).Applications• Single cell gel electrophoresis (Comet assay) (5, 6)• Alkaline elution (7)• Alkaline unwinding (8)Product SourceE. coli BL21 (DE3) strain expressing E. coli Endonuclease IV gene.Product Includes• Endonuclease IV (Nfo)• 10x Endonuclease IV reaction buffer
New England Biolabs, Inc. Antarctic Phosphatase – 5000 units
Antarctic Phosphatase (AnP) is a heat labile alkaline phosphatase purified from a recombinant source. AnP nonspecifically catalyzes the dephosphorylation of 5' and 3' ends of DNA and RNA phosphomonoesters. Also, AnP Hydrolyses ribo-, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs). AnP is useful in many molecular biology applications such as the removal of phosphorylated ends of DNA and RNA for subsequent use in cloning or end-labeling of probes. In cloning, dephosphorylation prevents religation of linearized plasmid DNA. The enzyme acts on 5' protruding, 5' recessed, and blunt ends. AnP may also be used to degrade unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis. AnP is completely and irreversibly inactivated by heating at 80C for 2 minutes, thereby making removal of AnP prior to ligation or end-labeling unnecessary.
New England Biolabs, Inc. beta-Agarase I – 100 units
B-Agarase I digests agarose, releasing trapped DNA and producing carbohydrate molecules which can no longer gel, purifying DNA fragments from gels.
INTACT GENOMICS INC T4 DNA Ligase 100,000 Units (2000 units/µl)
Intact Genomics T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. This enzyme joins DNA fragments with either cohesive or blunt termini and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids (1).Applications• Cloning of restriction enzyme generated DNA fragments• Cloning of PCR products• Next-gen library preparation• Joining linkers and adapters to cohesive or blunt-ended DNA• Nick repair in duplex DNA, RNA or DNA/RNA hybrids• Self-circularization of linear DNA
New England Biolabs, Inc. Enterokinase, light chain – 2560 units
Enterokinase is a specific protease that cleaves after lysine at its cleavage site Asp-Asp-Asp-Asp-Lys. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate.
New England Biolabs, Inc. RNase If – 5000 units
Ribonuclease If (RNase If) is an RNA endonuclease which will cleave at all RNA dinucleotide bonds leaving a 5'hydroxyl and 2',3' cyclic monophosphate. It has a preference for single-stranded RNA over double-stranded RNA. RNase If is a recombinant protein fusion of RNase I (from E. coli) and maltose-binding protein. It has identical activity to RNase I.
Sigma Aldrich Fine Chemicals Biosciences Alcohol Dehydrogenase from Saccharomyces cerevisiae | 9031-72-5 | MFCD00081305 | 1VL
Alcohol Dehydrogenase from Saccharomyces cerevisiae | 9031-72-5 | MFCD00081305 | 1VL
Sigma Aldrich Fine Chemicals Biosciences beta-Amylase from sweet potato | 9000-91-3 | MFCD00081391 | 1VL
beta-Amylase from sweet potato | 9000-91-3 | MFCD00081391 | 1VL
Sigma Aldrich Fine Chemicals Biosciences DNASE I RECOMBINANT RNASE-FRE
DNase I recombinant, RNase-free
INTACT GENOMICS INC Taq DNA Ligase 10,000 Units
Taq DNA Ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5′-phosphoryl and 3′-hydroxyl termini, using NAD+ as a cofactor. The ligation will occur only if the oligonucleotides are perfectly paired to the complementary target DNA and have no gaps between them; therefore, a single-base substitution can be detected. This product is active at elevated temperatures (45°C-70°C) (1, 2).Applications Allele-specific gene detection by using Ligase Detection Reaction (LDR) and Ligase Chain Reaction (LCR) (1). Mutagenesis by incorporation of a phosphorylated oligonucleotide during primer extension amplification(3).